FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3272429
Sequences flagged as poor quality0
Sequence length40-301
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT350.00229511641886.9506295
CTCGCGT1302.7066562E-7327.02832225-229
GGCGCCG4106.4623315E-4322.1623295
TAGGACG2358.5059827E-4281.0352295
CGCGAGT1657.070412E-8256.2753205-209
GAGCGTA2154.331533E-4192.79462110-114
AGCGCGC1650.0044240057169.7112185-189
TAGGCGT2450.008470798169.63351140-144
AACGAGG4000.0040495223165.10818295
GACGCTA2551.1971188E-6164.28159170-174
ATCGGCC2900.004135594142.93394115-119
GTTATCG1558.5308484E-4140.16747260-264
GCCGGTT3003.1577656E-9138.16946110-114
GCTCGAC1300.009617122130.91733225-229
GACCGTC3458.4312946E-5121.74934190-194
ACCAACG3600.0016897327117.93441220-224
GCCGCTT3902.4920155E-10113.44945285-289
TCGGAGG6107.8034645E-10108.26765295
AAGCGGG6151.7660295E-6107.387436295
AGCCTAT12653.4536573E-4106.7283290-294
Produced by FastQC (version 0.11.5)