FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3272429
Sequences flagged as poor quality0
Sequence length35-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA4006.1450986E-4327.62457295
ACACGCG1306.3504186E-4324.50824225-229
CTCGTAA2757.1988994E-4310.9877250-254
CGCGTTG1600.0010586816273.59164290-294
TGTCGTA2150.0026505457201.35643280-284
ACGCGTA900.0031292676190.5673250-254
GCGATAT2200.009273904189.46297175-179
GCGCGAT1550.0034358772184.67226255-259
ACTGCGT4550.0035090474183.21693175-179
CACGCGC1600.0039775786175.85406225-229
TCGCTCG1250.008502502175.09866290-294
GTCGTTT4850.0041804034172.80186205-209
CCGAAAA5102.6682937E-5169.30408275-279
TTCCGCC5150.0046509593166.7429255-259
TAGCGTC1700.0050487323162.38002130-134
CGACGGT1100.0062069246151.57037180-184
GGTTCGA2803.741836E-5150.86731230-234
AGGTTCG2854.9004233E-5150.65367255-259
GCGCGTC1404.5311122E-4149.0617195-199
CGGCCTA2901.6798153E-5148.4624265-269
Produced by FastQC (version 0.11.5)