FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3659475
Sequences flagged as poor quality0
Sequence length43-301
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA350.00104366382797.3745240-244
AACCGCG850.00246834781817.3794295
CGACGTA750.00455091431339.3982280-284
ATCGCGA750.0051207961262.7568150-154
TCGTCGA800.00557462171210.2152230-234
TCGTGCG800.00563697751203.5104215-219
CGAACGG1500.00768475931029.8484295
CGGGTAC2552.3090546E-5393.94058285-289
ACTTTCG4803.6290934E-4390.35693130-134
CTCGCGT1303.0456067E-7380.86108255-259
CACGAGA5605.4839446E-4340.0918175-179
CCGCTCG1905.8142806E-4334.12524180-184
GCTCGAC1556.3351716E-4324.75186285-289
CGCTTCC6508.5696654E-4293.00214185-189
TCGCGTT1151.0393887E-7287.02573255-259
CGCCGGC3750.0026537161253.93518185-189
CCGGAGT4100.005323885234.8313220-224
GACGTGT4002.8751007E-5234.21414125-129
GCCGGAA4304.2649554E-6233.61594285-289
GACACGA2900.0019571818222.77515230-234
Produced by FastQC (version 0.11.5)