FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3031994
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTGA2752.4248417E-588.8729290-294
TATCGAC2306.852133E-978.344124285-289
GTGCGAC2300.001508419677.19048280-284
TCGACAA2450.002158151270.52748270-274
CGAGTAG14300.054.8612253
AGTAGCT57450.054.21185
GAGTAGC36500.053.168324
CCGAGTA15550.051.790722
GTAGCTG57100.051.547456
GCTGGGA110350.051.1008729
TAGCTGG62200.049.5020147
CGACATG3600.00889623649.316143280-284
CCCGAGT18800.048.518461
CTACTCG10350.046.9155652
TACTCGG11100.044.6381533
GCTACTC27250.043.689161
AAAGTGC63300.042.5855644
CCAAAGT68800.042.5188942
AAGTGCT64800.042.2369845
CCCAAAG80500.042.2337951
Produced by FastQC (version 0.11.5)