FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3170139
Sequences flagged as poor quality0
Sequence length36-301
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG450.0079457781014.2942295
AAGTCGC2850.0022213887213.53561290-294
AACCCGC2700.0027518922198.8122205-209
GCGCCGC4150.0074794767142.30714280-284
GGCGATT3750.008120592138.46136145-149
CGTACGA650.008704466137.87839210-214
CGCGTTG952.9009141E-5136.52496140-144
GCGCTCA4150.008622151135.70328260-264
GCAACCG3850.008697293135.31906150-154
CGGACGG2200.008290231126.156204245-249
ATGCGCC2606.424979E-5106.461105240-244
CTCGCGT1906.5284094E-6105.31799285-289
CCGTATC2650.0018314999103.34318230-234
GCGAATG2700.002107846399.4061205-209
GAATGCG3006.717328E-494.607506265-269
GCGTCGC1707.8659394E-490.9422110-114
AACCGGC3500.00118903979.87125255-259
GGGACCA17550.001434463278.02263295
GGACCCG3902.3202358E-478.02262290-294
CGCGAGT1200.00396510976.934456240-244
Produced by FastQC (version 0.11.5)