FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3170139
Sequences flagged as poor quality0
Sequence length36-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA959.738123E-5606.54724285-289
CGTTGCG1008.69854E-4292.2063290-294
CGGCAAT2100.0011177636268.61642275-279
GCGTCGC1250.0052194213232.88208290-294
CATACGA2250.0017685619230.46643160-164
ACGACGT1200.0018104729228.7605245-249
GCGCGAC1350.0021363322216.44911290-294
TCGCGAT950.003584986182.10033160-164
GCTTCGC3200.0037001441180.06871285-289
CCGTTGG3400.004586495167.59686280-284
ACGATCG800.0098668905167.39664215-219
ATCGTTC3350.005390287158.79755200-204
CGAAAGT3950.0071813967144.26057280-284
ACCGCGG1950.0077886796140.463240-244
CGTAGGA3001.5405464E-4136.95143240-244
CCGGTAC1950.0033158762131.41405105-109
ACCGGGT2455.9224836E-5111.25341235-239
GGGTTCG2609.631631E-6100.90922180-184
CCGGTTC2959.458745E-1197.66439285-289
GCGTTGA2801.0565718E-597.0892230-234
Produced by FastQC (version 0.11.5)