FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528837
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA800.009342289132.20601265-269
AACCGTC2753.8335016E-581.03269290-294
CGAGTAG18400.077.980023
CCGAGTA20500.073.194182
AGTAGCT71400.071.710775
GAGTAGC46950.071.0127944
GTAGCTG75100.068.098476
TACTCGG12650.067.772913
TAGCTGG80300.066.431897
CTACTCG12950.065.088772
GCTACTC30600.064.8749161
GCTGGGA143000.064.65769
CCCGAGT24200.063.74771
TCCGAAA5101.2576254E-463.74267295
AAGTGCT66550.062.086275
AAAGTGC66550.061.54954
TTGGGAG95900.060.2815976
GCACTTT72900.059.2201961
CAAAGTG72750.059.168353
CCCAAAG82600.059.142841
Produced by FastQC (version 0.11.5)