FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802421
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTA900.007848787140.13684275-279
TAGGCGT3854.2145984E-966.388985280-284
CGCTACG1350.002776001366.19515245-249
GCGCTAC2750.002992553964.94359245-249
CGAGTAG13850.057.7019733
CCGAGTA15600.056.6119652
CTACTCG9950.053.9512252
GCTGGGA111750.053.3527539
ACGTAGC3700.006594998853.192066290-294
AGTAGCT59000.052.524555
GTAGCTG59600.052.1887136
TTGGGAG77300.051.841666
GAGTAGC36650.051.454754
TAGCTGG63800.050.6455467
CCCGAGT18250.050.6173821
TACTCGG10900.050.1098023
AAAGTGC61450.049.4947784
GCTACTC27650.047.8170621
AAGTGCT64350.047.6216285
CCCAAAG76400.046.7778051
Produced by FastQC (version 0.11.5)