FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2580384
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAA1808.106775E-490.25336290-294
CTACTCG15700.075.669142
CGAGTAG21250.072.5297853
AGTAGCT76500.070.1246265
GCTACTC35050.069.8597261
TACTCGG16350.069.786473
AACGCAT3953.211666E-668.54686290-294
TAGCTGG81500.067.580377
GTAGCTG80100.066.897096
CCGAGTA23200.066.207212
GAGTAGC50050.066.124344
AAGTGCT75700.065.262735
GCTGGGA157450.064.878489
AAAGTGC75250.064.620984
CCAAAGT81850.062.9691432
CCCAAAG91600.062.110871
GTGCGAC2600.003741862761.383316280-284
CCCGAGT26150.061.0470471
TTGGGAG100700.060.449396
CAAAGTG81700.060.323643
Produced by FastQC (version 0.11.5)