FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2580384
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCG600.0068376116146.7611145-149
CGAGTAG17850.082.0442663
CCGAGTA19600.075.3232
CTACTCG18600.073.588382
AGTAGCT71300.072.198245
GAGTAGC44600.071.042844
AAAGTGC70050.069.640424
GTAGCTG75350.069.198526
AAGTGCT71400.068.915145
TACTCGG20400.067.979533
TAGCTGG79750.067.5707557
GCTGGGA150500.066.5890969
GCACTTT85450.066.3959961
TTGGGAG112750.065.6019446
CCAAAGT78100.064.362312
CCCGAGT23750.063.4343761
CCCAAAG88400.063.165851
ACTTTGG92000.063.1536983
CAAAGTG78000.062.7638663
CACTTTG91200.061.5399932
Produced by FastQC (version 0.11.5)