FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3493860
Sequences flagged as poor quality0
Sequence length42-301
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG500.0032121081594.7338160-164
AATCGCG650.00489437671291.7716255-259
ACGCGTA700.0063552651133.6583150-154
GCGTACG800.00810297951003.9312195-199
TAGCTCG2650.0022947174324.21823285-289
CGATTAC2456.514006E-4321.59976125-129
CTATCGC2908.8863267E-4289.90103255-259
ATAGGCG3154.4265988E-5271.3527280-284
TTGCGGG5300.0048393724246.02441295
CCCGTAG3305.9519945E-5243.37724195-199
CACGGTT5503.7267455E-8237.07808295
GTAACTA11500.0018392276226.77034295
ACCGGGT3852.6061494E-4218.0913255-259
CACTTCG6650.0043731756196.0796295
GGAACGT4958.547431E-9171.79572265-269
TCGAACC3100.0042641424171.75134160-164
CTCCGTA4653.0586307E-7170.25192140-144
TCCAACG5002.7086164E-4170.07774270-274
TGCTTCG5000.005588664156.84056120-124
CGAGGGG8351.1188846E-5156.15921295
Produced by FastQC (version 0.11.5)