FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3493860
Sequences flagged as poor quality0
Sequence length41-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG500.0012000942607.208295
TACGCGT500.00290598071676.5336270-274
CGCGTAT600.0041842811397.1113270-274
ACGCGTG1300.0081093431002.7723295
CGTCGCA905.1582616E-4347.90164120-124
TCCGGGG8301.1514203E-8314.12143295
GCGCTAC2750.0031080563307.15714285-289
TCGTAGG2708.331954E-4296.2206190-194
CGGTGCG2903.1051756E-4291.2697285-289
CGCGGCA3100.0075885467269.0464255-259
TAGGCGC3100.0075885467259.8818220-224
GGGTCGA3257.4792246E-5258.58292280-284
ATCGCGG1300.0013864147250.07771230-234
AGTCGTT3400.001389251249.70996290-294
ATCGGGT3700.0023925363212.56685135-139
TTGATCG3750.0026846519210.23201145-149
CCGACAT3850.002358525209.25548215-219
CGGTTGT4054.25013E-4208.56349285-289
CCCAACG4054.2970458E-4207.5048280-284
CTACGCC4051.527751E-7207.5048280-284
Produced by FastQC (version 0.11.5)