FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856796
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19100.074.432123
AGTAGCT71350.069.453255
CCGAGTA21250.067.738732
GAGTAGC46750.067.0575564
CTACTCG16200.066.824712
GTAGCTG76300.065.8246846
GCTGGGA154800.063.559889
TAACGGG2450.063.550495295
TAGCTGG81450.063.5136457
TACTCGG17000.063.3323363
AAGTGCT76600.062.440855
CCCGAGT23850.062.3603251
AAAGTGC77300.062.026074
GCGACGC2600.003661121261.72366285-289
GCTACTC38850.060.9465031
CCAAAGT84850.058.8854642
TTGGGAG111900.058.70976
GCACTTT83800.057.0070041
CCCAAAG96800.056.8490371
CAAAGTG85750.056.743363
Produced by FastQC (version 0.11.5)