FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970572
Sequences flagged as poor quality0
Sequence length49-301
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGTCG2354.4577735E-4364.89618245-249
CGTACAC2605.9804355E-4330.8019250-254
CGCGCCG2556.1940926E-4326.96304130-134
CCGGTCT3650.0017118683232.83865230-234
TTAGCGG2053.0130625E-4212.65486275-279
GTCGTAC1050.0025394822204.32126245-249
TGGCGAC4300.0029478346194.17978165-169
GACCGAT1100.006123008191.72583200-204
TAGGCGT2701.3544418E-4162.45073285-289
CGGGTCG1302.6900123E-4161.99063195-199
GGTCGTA1900.0063335705150.51486245-249
CCCGTAA2850.0035115315150.21443235-239
GCGGGTA3057.9817255E-8143.80884285-289
GCGGTCC3201.3799217E-7136.8579280-284
GGGATAC6250.008670238135.37433210-214
GCGGGTT3103.0881398E-5135.06741175-179
CTACACG3102.9641027E-5134.47675125-129
ATACCGG1700.001636284129.00499285-289
TCGCGCC3352.7623391E-6124.62284155-159
AATCGGG3450.0011841601123.168274230-234
Produced by FastQC (version 0.11.5)