FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3376412
Sequences flagged as poor quality0
Sequence length39-301
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG901.9393628E-5306.60696235-239
TGCGCGG1858.4779836E-4294.61905225-229
CGTAGGA3009.7257325E-6273.65436230-234
GCGCACG2100.0012484256258.90292220-224
GTTCCGG4000.0020391706219.65323295
GCCGGAC2550.0021079606217.33858240-244
ATTCGTA4100.0021955902214.29584295
ACATCCG4053.704933E-5195.71704125-129
GTGTCGT2850.0029257375194.78848245-249
CTCGCTA1950.006897819146.2892265-269
CCCGGAC4000.0077825096140.44324255-259
CGAAGGG4250.008542877136.12761275-279
TACGTAA4100.008835458134.60794235-239
TAATCGG2255.5193255E-4117.24219105-109
AAGTCGG3950.0032622057111.21683295
GACGGAT2950.004245853100.078926290-294
GATCGCG3008.474265E-590.84088225-229
ACGTAGG3250.0081829386.42661255-259
AGGTCGA3107.955746E-485.36853140-144
GCGCGGT3203.6658737E-585.163315225-229
Produced by FastQC (version 0.11.5)