FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3376412
Sequences flagged as poor quality0
Sequence length35-301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT1151.7170372E-4502.01038285-289
CGGCGCG1801.8495064E-4489.48822295
GTCGCGT652.4500838E-4446.04764290-294
CCGCGCA1554.54094E-4362.88452260-264
TGTCGCG855.473296E-4341.09525290-294
GTCCGAT1907.6474197E-4304.92114290-294
ACGCTAC1907.954953E-4300.93698275-279
GTCGCGC750.0012565732258.47754290-294
ACGAATC2300.0017545379231.08737175-179
GCGACGC1350.0041554356205.67892250-254
CGGATGT4003.5126373E-5198.34923160-164
CGCGGAT904.093688E-5191.05939275-279
TAGTACG1450.0035238366183.12793170-174
ATCACCG3350.0044926736168.76952265-269
TGTGCGG5250.0045622233167.82454295
GTCGGAC1800.005578882157.04941265-269
CGAACTA1850.0056138965156.71945290-294
GCCCGCT3800.006250237151.1267280-284
CGCTCGA800.0066493703148.13228290-294
ATAGCGC1950.0038057629139.67522230-234
Produced by FastQC (version 0.11.5)