FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3053239
Sequences flagged as poor quality0
Sequence length38-301
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCG955.5650584E-5730.8585295
CACGTTA3253.2152522E-5202.82153265-269
TCCGACA2150.003159818189.87328210-214
CGAGCAT2750.004535214168.25441285-289
ACGTAGA4150.0046073603167.30498295
GCGATCG800.004952987163.45912115-119
TCAGTCG2650.005140389161.36295250-254
CGTCAAG2600.0062843775150.88535110-114
TCCGTAC2051.13779795E-4147.80153200-204
GACGTCT3150.008710144135.27045245-249
TAGCGAG2250.00887768897.654816265-269
TCGGAGC4558.601953E-495.814064260-264
ACGTTAG2550.00727308188.22218275-279
CCGTATG2608.390401E-586.5256275-279
CCGTTGG4507.4639975E-477.14618295
CCGTACC2650.001584470376.23319200-204
CGGCCTA2951.5127818E-569.33183215-219
CGCGCCT3152.8103384E-569.19904260-264
TATAGCG3051.1576121E-665.98501195-199
TACTACG3158.3560887E-465.2839220-224
Produced by FastQC (version 0.11.5)