FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3053239
Sequences flagged as poor quality0
Sequence length38-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG200.0080117631010.513205-209
TACGCGT405.520556E-4340.1908215-219
TACGACG750.0011937837262.93213155-159
TTACGAA3450.0028843877195.66269295
CGCGAGT1352.9635354E-4166.48877285-289
GTCGCTT2500.0048084385165.00887225-229
CTAATCG1350.0076129646146.6504165-169
CGGCGAC1350.007122514144.74461105-109
TCGGTCT3100.0074781766142.34665275-279
CTCGCGT1506.027849E-4141.18716250-254
CGCCGGA1600.007972542139.3811280-284
GCCTGCG5150.009558373131.07501295
TCGCGTT1050.0017721616103.959435270-274
GCCCGTA1900.0029227117103.382256135-139
GCCCGAT2100.0056849676102.28866260-264
CGTGTCG2103.0297696E-4100.84797250-254
TTCGGCG1000.00133391899.90985185-189
TAGGACG2150.006670811795.93539225-229
GGCGTAG2306.0496345E-494.06588265-269
GGACCGA3654.6744753E-792.47071295
Produced by FastQC (version 0.11.5)