FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793363
Sequences flagged as poor quality0
Sequence length35-301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG15100.068.5387043
CTACTCG15850.067.874212
TACTCGG15550.066.931293
GCTACTC36300.064.284611
CGTACGA859.286263E-562.662437235-239
GAGTAGC38450.062.5175254
AGTAGCT63350.062.4136665
CCGAGTA16500.061.6568832
GTAGCTG63950.060.5628176
TTGGGAG96650.059.0794646
GCTGGGA129500.058.7629479
TAGCTGG69600.057.339937
AAAGTGC66150.057.2045754
AAGTGCT66200.056.8993765
CCCGAGT19050.056.795961
GGAGGCT105500.056.4284939
GCACTTT76450.056.3042341
ACTTTGG79950.054.5581863
CAAAGTG72200.053.854753
CCAAAGT74450.053.7164882
Produced by FastQC (version 0.11.5)