FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741339
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG21800.076.962933
AGTAGCT82700.071.895425
CCGAGTA24300.071.828152
GAGTAGC51350.070.5941544
GTAGCTG86050.069.309956
CTACTCG16750.068.9803852
TACTCGG17150.068.5527043
AAAGTGC78050.067.470364
TAGCTGG90900.067.026527
CCAAAGT84900.066.0915152
AAGTGCT81450.064.954635
CAAAGTG84150.064.9083
GCTGGGA175850.064.802469
GCTACTC38600.064.178241
ATACGTA3450.003247398963.614796295
CCCAAAG97850.062.256241
CCCGAGT28250.062.232561
GCACTTT83600.061.7650871
TTGGGAG109650.061.037826
ACTTTGG88450.058.637183
Produced by FastQC (version 0.11.5)