FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4215403
Sequences flagged as poor quality0
Sequence length46-301
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA600.00444529951355.8049120-124
AATCGCG700.0059522051171.6245155-159
TACCGGG2307.903929E-4361.75995235-239
CCCGTAG3950.0038635645333.98328295
ACCCGAC2758.371315E-4295.81198125-129
CTAACCG3050.0063434835271.6753220-224
GCGCCTT3200.009390266270.68515270-274
ATAAGCG3150.0012119049261.43027175-179
CCGCGTT1600.0011801377257.87494185-189
ACCGTAG3650.0020910753224.23657145-149
TACGGCA3750.0026173422220.05328195-199
GCCGACA3900.0034773736216.94638260-264
CGGCCCA7750.0021507533215.61671250-254
GGACCGA3900.0036218627211.58969185-189
CGTAACA4050.0026838575200.45338100-104
GGCGTAG4109.840733E-9200.03346155-159
TAAGCGG2800.0028701213196.0727175-179
GGGGCGA4600.0013441544189.9437285-289
TTAGGCG3000.0032263303188.5506260-264
GCCTATC9003.754394E-9187.23021255-259
Produced by FastQC (version 0.11.5)