FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4215403
Sequences flagged as poor quality0
Sequence length46-301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCG550.00129212962513.229295
TCGCGTA300.0039469381439.5594270-274
CCGTACG1000.00427058251382.2759295
GTCGCGA650.0050885151267.1776200-204
TTACGCG700.00535439471235.2435275-279
TACGCGT450.008859367960.745275-279
GATCGGG3507.371491E-6394.936295
TAGGCGT3059.496762E-8283.4985275-279
GCGATGC3150.0012037145262.02133215-219
AAGCCGT4801.3135361E-5253.73332115-119
CGGCCTA3551.6079866E-9245.16109290-294
GGTTGCG3601.1878001E-9241.7561290-294
GCGTGTA3450.0013421464236.7907150-154
GCTGACG5901.4978692E-4234.28404295
CCGATAG1851.5422648E-4233.44208270-274
ACCCATA11900.0017132848232.31528295
CGCGGGC3701.4551915E-10223.53409225-229
GGCGCAA2650.002554837203.84898100-104
CCGTGAG8358.0878363E-4199.75635250-254
ATGCCGG4203.518926E-6198.56734250-254
Produced by FastQC (version 0.11.5)