FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464914
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17150.078.163173
CCGAGTA17700.076.429192
AGTAGCT64400.073.346975
CTACTCG17150.072.067882
TACGCGA350.001994932871.95397190-194
GAGTAGC39700.071.877044
ACGATTG2556.9917536E-571.78466265-269
GCTACTC40700.071.4810941
TACTCGG17300.070.732133
GTAGCTG65900.070.4727556
TAGCTGG70450.068.044437
TTGGGAG107250.067.964296
GCACTTT80900.066.2967761
GCTGGGA139450.065.945169
AAAGTGC66850.065.316014
ATCGGTT3300.002946897265.191295
CCAAAGT73300.064.762432
CCCAAAG81800.063.988321
ACTTTGG84000.063.5404473
AAGTGCT68250.063.261765
Produced by FastQC (version 0.11.5)