FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334430
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCG1257.535672E-5163.92873295
CGCGAAT600.006838146146.75641280-284
GCGCTAA950.0072657745143.79715295
TCGATAC1406.7986804E-494.34341280-284
AGTAGCT73250.075.290525
CGAGTAG20000.075.2049563
CTACTCG14050.073.890112
GAGTAGC47100.072.761394
GTAGCTG75200.072.747416
TACTCGG15150.071.632753
TAGCTGG80100.070.277987
CCGAGTA21250.070.134542
AAGTGCT65300.068.420725
AAAGTGC66100.068.16874
GCTGGGA145850.067.576119
TTGGGAG93450.066.010826
GCTACTC32550.064.979171
CCAAAGT73600.064.4693762
CCCAAAG80200.064.263041
GCACTTT74150.063.578111
Produced by FastQC (version 0.11.5)