FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334430
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG17750.082.984532
AGTAGCT67250.080.924455
CGAGTAG19150.079.570113
GCTACTC39800.077.7112051
TACTCGG18150.077.657573
CCGAGTA19800.076.957962
GTAGCTG68950.076.638236
GAGTAGC43600.076.460324
TAGCTGG75050.074.41217
CACGATT2700.001881024773.00737295
GCTGGGA146900.071.378929
TTGGGAG110850.070.015146
GCACTTT81250.069.489021
CCAAAGT77800.068.549952
AAAGTGC70900.068.513874
AAGTGCT72200.068.252065
ACTTTGG84950.066.741443
CCCAAAG85800.066.692241
TTTGGGA95600.066.690975
CAAAGTG76650.066.099513
Produced by FastQC (version 0.11.5)