FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262466
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGTA2052.1528367E-591.02995280-284
CGAGTAG17100.076.281143
AGTAGCT64900.073.727095
CCGAGTA17900.072.1227342
GTAGCTG68100.070.535016
GAGTAGC42300.070.442954
TAGCGGC2708.104696E-569.673096285-289
TAGCTGG70900.068.882947
TACTCGG13550.067.979483
GCTACTC31100.067.469111
CTACTCG13400.066.699042
AAAGTGC63950.066.412074
GCTGGGA135550.065.719669
AAGTGCT67150.065.3646855
CCAAAGT69150.063.817432
CCCGAGT21800.062.364331
CCCAAAG78950.061.9616131
CAAAGTG69800.061.9088333
ATACGAT2300.00387319560.851543280-284
TTGGGAG91600.059.4581346
Produced by FastQC (version 0.11.5)