FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2262466
Sequences flagged as poor quality0
Sequence length35-301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTT1102.4534442E-4121.896065265-269
AGTAGCT66600.079.158355
CGAGTAG17150.078.894773
GAGTAGC40250.077.995894
GCTACTC36250.075.858921
GTAGCTG69550.075.462046
CCGAGTA18100.074.410892
CTACTCG15250.074.137832
TACTCGG15250.072.924073
AAAGTGC67500.072.6926654
TAGCTGG74050.072.314637
AAGTGCT68250.072.173475
CCAAAGT75600.071.424672
GCTGGGA140450.070.680899
TTGGGAG104100.069.538326
CAAAGTG73050.069.437033
GCACTTT75250.068.0773541
CCCAAAG88300.066.7641
CCCGAGT20700.065.974571
ACTTTGG78750.065.901753
Produced by FastQC (version 0.11.5)