FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623027
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18900.072.220663
AGTAGCT72100.068.406295
CCGAGTA20700.067.063992
GTAGCTG73850.065.22456
GAGTAGC48250.065.003084
TAGCTGG79200.062.7334947
AAAGTGC68700.062.1665344
AAGTGCT70500.061.870065
GCTGGGA145100.061.794219
TACTCGG14200.061.51983
CTACTCG13400.060.887042
TTGGGAG96550.060.131276
CCAAAGT74300.059.9036672
GCTACTC33600.059.8123551
CCCAAAG82250.058.049221
CCCGAGT24950.057.604671
TCGAAAT4050.00491156757.30216295
CAAAGTG77650.055.704313
GCACTTT81300.055.5065041
ACTTTGG81050.054.7148323
Produced by FastQC (version 0.11.5)