FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623027
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19650.079.4393163
CCGAGTA20200.078.479112
AGTAGCT68550.075.142655
CTACTCG17000.071.814482
GAGTAGC45500.071.5527954
CCCGAGT22950.070.945481
GTAGCTG72900.070.916916
GCTACTC41400.070.87931
TAGCTGG78350.068.7121667
TACTCGG17650.068.48153
AAAGTGC71300.066.78824
GCTGGGA150400.066.606119
TTGGGAG106400.065.8888556
CCAAAGT79650.065.733452
GCACTTT82800.064.9360051
AAGTGCT73950.064.4806755
CAAAGTG79250.063.382763
ACTTTGG84150.062.5070043
CCCAAAG92950.061.701431
TTTGGGA95750.061.0921755
Produced by FastQC (version 0.11.5)