FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349118
Sequences flagged as poor quality0
Sequence length35-301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG1501.6370905E-10103.793495250-254
CGAGTAG17850.083.819423
AGTAGCT67500.080.01555
GAGTAGC43450.079.22524
CTACTCG17900.079.113632
CCGAGTA19000.078.746132
TACTCGG18800.077.618833
TACCGAC900.00164139675.57435265-269
GCTACTC40050.075.042081
GTAGCTG71250.074.949386
CCCGAGT21550.073.446891
TAGCTGG74250.072.189987
ATTCGCG650.001638165271.44179285-289
TTGGGAG103250.070.39246
GCTGGGA141250.068.9379
AAGTGCT71200.068.072715
AAAGTGC70650.066.5049064
CCCAAAG83350.066.130981
CCAAAGT74950.065.71962
ACTTTGG80250.065.215443
Produced by FastQC (version 0.11.5)