FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3157348
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCG1857.53158E-7178.61047290-294
CGCGAAT700.0055950456156.9402695-99
CGTATAT3902.67828E-8153.03268295
CTACGCG850.0071931374144.28918165-169
CTAGCGT2154.95577E-576.95291290-294
TACGGGA3150.002093372471.066025255-259
GGTACGC1100.001686408469.737265-269
CGATGAC4951.109599E-465.38135285-289
CGATCGC1200.00370115660.670853245-249
TAGCGTA1851.7130736E-459.904076290-294
CGATAGC1850.00453760458.472134240-244
TTGTCGA3153.871853E-450.784622280-284
TACTCGG11150.049.260793
TCGCGGG1550.00851926545.977287235-239
CGAGTAG12900.045.5887033
AGTAGCT51000.045.5811125
CCGAGTA13300.045.2223552
GCTGGGA102400.043.8860179
CTACTCG11450.043.774192
GTAGCTG52300.043.599476
Produced by FastQC (version 0.11.5)