FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3157348
Sequences flagged as poor quality0
Sequence length35-301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG400.0062540933151.21402160-164
TAGCGGC3000.001462078277.79315285-289
TACGCTA1405.2213363E-755.312283190-194
TACTCGG13050.055.218543
CTACTCG12800.054.996992
CCGAGTA13600.050.9467282
CGTTCGT1555.1441876E-450.79926290-294
CGAGTAG12800.050.2342723
AGTAGCT51200.049.1617625
GCTGGGA103250.048.6252379
ACCGTAG2450.00968786748.267227290-294
GTAGCTG51550.048.081226
CCCGAGT16950.047.4262961
GAGTAGC31800.046.894884
GGAGGCT86550.046.2137769
TTGGGAG83350.045.8363726
TAGCTGG55950.044.9060557
CGCTAGT1607.424882E-544.721516245-249
CCCAAAG74800.044.6927031
AAAGTGC61200.044.0235524
Produced by FastQC (version 0.11.5)