FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2797924
Sequences flagged as poor quality0
Sequence length39-301
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT300.008263951994.7708295
TCGGTCC2001.1727574E-5261.19733155-159
TACGAGT1400.001233664259.9779225-229
TTGCGCG350.0014452115246.71498110-114
TTCGCGT1550.0018284199227.9649195-199
TTCGACC1850.0029426194194.45236210-214
GTCGTAT1203.7519876E-6145.83928165-169
TAAGCGT2550.008561448136.06322140-144
TCGCGTT1350.009617323130.91028195-199
CGTGTCG1556.8419107E-4111.644714130-134
CCGGTTA1752.7084661E-6109.95611265-269
CGTTCGT1902.7248502E-494.667595210-214
TACCGGG2153.274181E-1180.38814115-119
GATCGCG2703.763489E-971.96411270-274
CGGGACG2450.005746422371.2077160-164
GCGATAG2708.3976425E-570.05746255-259
ACGTTCG959.358114E-468.33548270-274
GTCCAAT9050.002616630467.1268290-294
CCCGGTT2750.002166614462.770973105-109
GCGCGGT3251.073422E-762.307438290-294
Produced by FastQC (version 0.11.5)