FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013818
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG700.00884001226.8560476
GCCGTAC1401.8980714E-523.5549221
AGTAGCT43400.023.4990435
CGTCGAG1852.7551687E-722.9183041
GTAGCTG44700.022.6053416
CGAGTAG11050.022.5420673
GAGTAGC28650.022.473784
GCTGGGA81150.022.066019
CCGAGTA11150.021.9183882
CCCGAGT14850.019.9859961
TAGCTGG53650.019.9730917
TTGGGAG58300.019.5086386
CTACTCG8950.019.4293752
GGAGGCT59150.019.2286159
TACTCGG9800.018.7033183
CAAGTAG22950.018.4306223
GCACTTT47050.018.1230241
GCTCGAT1851.621573E-417.8253481
GGGAGGC72650.017.4665978
CCCAAAG53750.017.4415991
Produced by FastQC (version 0.11.5)