FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013818
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT44550.023.522125
CGCCGTA1802.1601772E-723.5035594
CGAGTAG11850.023.3958343
GTAGCTG44800.023.1810726
TTGGGAG60200.023.1054526
GCTGGGA80500.022.4147019
GGAGGCT64950.021.9933859
GCTACTC27400.021.954781
CCGAGTA12500.021.8026072
GAGTAGC30350.021.6764744
TACTCGG10800.021.319513
GCGATAC1554.2435357E-521.224431
TAGCTGG52250.020.8644317
CTACTCG10850.020.7874852
GGGAGGC79200.019.8754658
CCCAAAG53950.018.9904291
AAAGTGC43300.018.6663684
CTCGGGA13900.018.5937585
AAGTGCT45000.018.4833225
CCAAAGT49550.018.2074032
Produced by FastQC (version 0.11.5)