FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11241367
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG39750.064.968523
TACTCGG56750.064.679413
CCGAGTA42800.064.014212
CTACTCG57750.061.2894442
GAGTAGC103600.056.4980774
GCTGGGA396550.055.6451349
GGAGGCT339250.054.9597179
TCGGGAG74500.052.514226
CTCGGGA71800.052.122435
CCCGAGT61100.051.8251531
TTGGGAG282800.050.7948886
AGTAGCT180350.049.64185
GTAGCTG185250.048.6410986
ACTCGGG77550.048.5990754
CGGAGGT78000.047.2193377
AAGTGCT286800.047.2132765
TAGCTGG201400.046.8577277
GTGCTGG283700.046.8475387
CCCAAAG339800.046.786771
AAAGTGC285250.046.645784
Produced by FastQC (version 0.11.5)