FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3995674
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGAG73050.032.2356876
CGAGTAG12850.031.4859733
CCGAGTA13150.031.4855562
AGTAGCT47600.030.2411445
GAGTAGC31800.029.7339554
GCTGGGA91300.029.5979849
CGTCGAG1602.0863808E-929.442421
CCCGAGT16600.028.9457971
GTAGCTG49000.028.8728626
GGAGGCT69000.028.6746469
GGGAGGC91500.028.405458
TTCGGCG1005.2946285E-528.2250695
TACTCGG10700.027.2602423
TAGCTGG57100.027.2451737
GCACTTT69850.026.9765871
CTACTCG10200.026.2923342
TTTGGGA77950.026.0706215
TCGCGTT1458.592615E-725.9540865
TGGGAGG95800.025.6095947
GCTACTC29150.025.5335921
Produced by FastQC (version 0.11.5)