FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3995674
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG12300.041.6587223
CTACTCG12250.040.677512
CGAGTAG13600.040.4418953
CCGAGTA13950.040.1011962
TTGGGAG81250.036.2558986
CCCGAGT18400.035.0278661
GCTACTC30000.034.81311
AGTAGCT49650.034.745085
GAGTAGC32200.033.7237054
CCGTACG702.1737887E-433.603381
CGGAGGT16650.032.7381677
TCGGGAG16400.032.6584436
GCTGGGA94900.032.6338169
GGAGGCT75750.032.3224079
GTAGCTG52700.032.094196
GGGAGGC105100.031.1207288
GCACTTT70650.031.0967981
TAGCTGG55450.030.5078457
CTCGGGA15300.030.1079465
AGGCGGA24800.029.75964
Produced by FastQC (version 0.11.5)