FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10138460
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG60850.072.7383043
CTACTCG63200.068.4004752
CGAGTAG43500.067.4810943
CCGAGTA46450.066.75852
GCTACTC153800.064.672311
GAGTAGC111600.064.197174
GCTGGGA428450.061.968049
AGTAGCT183400.061.586345
GTAGCTG189350.059.743356
GGAGGCT362600.059.1169669
CTCGGGA75550.057.8888665
TCGGGAG78350.057.736426
CCCGAGT58350.057.1597061
ACTCGGG79600.054.778524
TAGCTGG215700.053.3579647
AAAGTGC293350.053.344944
TTGGGAG292200.053.2072836
AAGTGCT300650.052.617585
GTGCTGG295700.052.5106357
AGTGCTG303100.051.4671486
Produced by FastQC (version 0.11.5)