FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10167175
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG54250.078.655053
CCGAGTA56000.077.527842
CTACTCG83300.076.669782
TACTCGG85350.075.185633
GCTACTC196200.072.217931
GAGTAGC139500.070.327134
AGTAGCT226250.069.034065
GCTGGGA549900.067.705929
TCGGGAG103250.066.6233756
GTAGCTG236950.065.961486
GGAGGCT461250.064.3282859
CTCGGGA103650.063.249975
CCCGAGT71450.062.887431
AAGTGCT353650.062.8710635
AAAGTGC355350.061.6208424
TTGGGAG351750.061.545356
ACTCGGG105150.060.649014
CCAAAGT386200.060.030192
TAGCTGG273800.059.257487
CCCAAAG427800.059.115851
Produced by FastQC (version 0.11.5)