FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3334640
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG8400.038.6477782
CCGAGTA11250.037.6395762
CGAGTAG10600.037.2811283
TACTCGG9500.036.645763
AGTAGCT41050.032.7749755
GAGTAGC25450.032.162594
TTGGGAG64800.032.130276
GCTACTC23650.032.0660321
GTAGCTG41800.032.0445486
GCTGGGA78550.031.7094869
CCCGAGT15350.030.0724071
GGAGGCT60850.029.9661339
TAGCTGG47050.029.8669347
GGGAGGC78700.029.202048
CGCGTAT653.3382457E-728.92052510-11
GCGCGTA1154.202422E-628.6061739
TCGGGAG13700.028.4736486
CCCAAAG57450.026.8926941
GCACTTT60550.026.2937811
TTTGGGA70000.026.1421385
Produced by FastQC (version 0.11.5)