FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10057960
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA42500.063.3348582
CGAGTAG41200.061.8106083
TACTCGG52300.060.583493
CTACTCG51500.060.1511342
GAGTAGC106150.055.6276474
GCTACTC130000.054.4780541
GCTGGGA385900.052.726279
AGTAGCT174200.052.0106245
GGAGGCT320000.051.4975789
CCCGAGT62350.051.0089651
TCGGGAG69950.050.992346
GTAGCTG176000.050.9081086
CTCGGGA65150.049.2363475
TTGGGAG270950.047.923526
TAGCTGG198950.046.416777
ACTCGGG71650.045.0437854
AAAGTGC257000.044.1947334
CGGAGGT67100.044.1842587
GTGCTGG262250.043.6232267
AAGTGCT266050.043.5772135
Produced by FastQC (version 0.11.5)