FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10057960
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG56150.065.868123
CTACTCG58400.061.4248772
CGAGTAG39100.060.102743
CCGAGTA41650.058.3089872
GCTACTC139150.055.8072171
GGAGGCT328750.055.280549
GCTGGGA386250.054.5399369
GAGTAGC100250.053.4125564
TCGGGAG74650.053.2978866
AGTAGCT165550.052.4635775
CTCGGGA70750.051.990745
TTGGGAG279050.051.145796
GTAGCTG171300.051.1134226
CCCGAGT56950.050.3580931
ACTCGGG76050.048.804324
TAGCTGG196350.047.62677
AAAGTGC261800.047.3070564
AAGTGCT265500.047.1592145
CCCAAAG316950.046.4815561
GTGCTGG267100.045.8649757
Produced by FastQC (version 0.11.5)