FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2910109
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG17550.068.690313
CCGAGTA19300.064.9965442
AGTAGCT70000.062.451115
GAGTAGC44150.059.496124
GTAGCTG71150.059.4798556
GCTGGGA139750.059.2939459
TAGCTGG75650.057.199797
CCCGAGT23350.056.188061
AAAGTGC72450.054.9971544
CTACTCG15150.054.640172
AAGTGCT73500.054.2114875
TACTCGG16200.053.6567763
CCCAAAG88050.053.4538271
CCAAAGT81200.052.9333082
GCTACTC35450.052.6399041
CGTAAAC3503.581082E-451.593563285-289
TTGGGAG90550.051.4311946
AGTGCTG76700.050.3786
CAAAGTG81750.050.060013
GGAGGCT95400.047.4863559
Produced by FastQC (version 0.11.5)