FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2910109
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG16150.071.914282
TACTCGG17250.068.433373
AGTAGCT65850.066.60915
CGAGTAG16800.066.110873
GAGTAGC42900.063.9157524
GTAGCTG67350.063.9069756
GCTGGGA144000.061.9771359
CCGAGTA18150.060.4931372
TAGCTGG74350.060.3798037
GCTACTC39250.059.8457221
TTGGGAG100450.058.7745256
AAAGTGC78300.058.364964
AAGTGCT80400.057.7128685
CCAAAGT84800.057.4032062
CCCAAAG92900.057.1979941
GGAGGCT107100.056.8783269
CAAAGTG83700.055.9596523
AGTGCTG83500.055.2718626
CTCGGGA21900.053.3316545
GCACTTT84350.053.2114871
Produced by FastQC (version 0.11.5)