FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3599099
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG15700.057.4784772
AGTAGCT64600.054.4937825
CGAGTAG17850.053.1876533
GAGTAGC40550.052.853244
GTAGCTG65700.052.508356
TACTCGG17050.052.3753433
CCCAAAG92150.051.9572141
GCTACTC35750.051.8338661
AAGTGCT76150.051.659865
AAAGTGC76300.051.5583044
CCGAGTA18400.051.0876432
CCAAAGT83800.050.4219252
GCTGGGA145600.050.1943249
TAGCTGG73050.049.477057
TTGGGAG100200.048.454076
CAAAGTG85150.047.469153
AGTGCTG83150.047.028246
CCCGAGT21700.044.2144131
GTGCTGG88550.044.213427
ACTCGGG20450.043.2078064
Produced by FastQC (version 0.11.5)