FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5938141
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT3000.050.3977745
CCGAGTA22450.045.251262
CGGAGGT26550.044.7437867
CGAGTAG22200.043.1644863
GCTGGGA170800.042.0262459
AGTAGCT87750.041.1058735
TTGGGAG139800.039.8093956
GAGTAGC56200.039.336514
GTAGCTG93300.038.660676
CCCGAGT33200.037.874231
GGAGGCT142350.036.617989
GGAGGTT73850.036.266518
TAGCTGG105450.035.981347
CTCGCGT4100.035.1269074
GGGAGGC179700.034.976758
CGCGTTG4350.034.7570846
TGGGAGG176600.033.063097
GAGGTTG98150.032.9358839
AGGCGGA42000.032.918934
CTACTCG23000.032.8919332
Produced by FastQC (version 0.11.5)