FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5938141
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA22550.048.2217372
CGAGTAG20750.046.493593
CGGAGGT30300.045.383417
AGTAGCT91450.045.1891945
TACTCGG27800.044.5440833
TTGGGAG140150.044.5381746
GAGTAGC54000.043.5973784
GTAGCTG96000.043.4973876
GCTGGGA187350.042.693999
GGAGGCT146950.041.5953259
TAGCTGG107050.040.7560737
TCGGGAG32100.040.5956886
GGGAGGC182200.040.4234738
CTACTCG29350.040.2390982
CTCGGGA30950.039.5452845
CCCGAGT31350.039.3230131
GGAGGTT77200.039.075378
AGGCGGA43050.038.130114
TCGCGTT2109.094947E-1237.712025
GCTACTC66950.037.4728621
Produced by FastQC (version 0.11.5)