FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3405606
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7700.043.3502433
TACTCGG9100.040.8140373
CTACTCG8550.040.6901662
CCGAGTA8350.039.975672
CGGAGGT12100.037.300637
GCTACTC21600.035.707081
GCTGGGA63800.035.441859
GGAGGCT59100.035.0786679
CCCGAGT12350.034.652831
GGCGGAG17150.034.5401275
TTGGGAG57000.033.3224376
CTCGGGA11750.032.4089285
AGGCGGA19050.031.589224
GGGAGGC73950.031.405548
GCGGAGG14950.030.8187586
TAGCTGG39900.030.5180767
AGTAGCT36400.030.3517655
CCCAAAG55500.030.2509821
TCGGGAG12650.030.1040366
ACTCGGG13350.029.9337774
Produced by FastQC (version 0.11.5)