FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3637725
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGGT13700.032.189977
CCGAGTA9050.032.1602332
TACTCGG7900.032.086243
CTCGGGA9350.031.6286835
CGAGTAG9150.030.780973
CTACTCG8150.030.5277162
AGGCGGA15250.029.8575444
GCTACTC21500.029.7986641
GCTGGGA59800.029.7350859
TCGGGAG10300.029.1463626
GGAGGCT53150.029.0418179
CCCGAGT12550.028.1523021
GGCGGAG16800.027.661655
GCGGAGG15650.027.5749746
GGAGGTT34000.027.4595498
TTGGGAG50900.026.9095656
GTGCTGG40250.026.109317
GGAGGCG16950.026.03362
GAGTAGC21000.025.9293044
GGGAGGC59000.025.7639378
Produced by FastQC (version 0.11.5)